Listeria monocytogenes-reactive T lymphocyte clones with cytolytic activity against infected target cells.

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Listeria monocytogenes-reactive T lymphocyte clones with cytolytic activity against infected target cells

Lyt-2+ T cell clones were established from Listeria monocytogenes-infected mice. The clones secreted IFN-gamma after stimulation with spleen cells from L. monocytogenes-infected mice plus IL-2. Spleen cells from normal mice were not stimulatory. Furthermore, cloned T cells lysed L. monocytogenes-infected macrophages. Cytolysis was antigen-specific and H-2K-restricted. These findings suggest a r...

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Abnormal cytolytic activity of lymphocyte function-associated antigen-1-deficient human cytolytic T lymphocyte clones.

The involvement of the lymphocyte function-associated antigen-1 (LFA-1) membrane molecule in cytolytic T lymphocyte (CTL) interactions with lymphoid target cells was investigated using CTL clones derived from two patients with a heritable deficiency of LFA-1. LFA-1 surface expression on the CTL clones was 1% of the normal level of LFA-1, unchanged with prolonged culture, and identical on 14 dif...

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Recognition of influenza-infected cells by cytolytic T lymphocyte clones: determinant selection by class I restriction elements.

The fine specificity of virus recognition by influenza A/PR8/34(H1N1)-specific cytolytic T lymphocyte (CTL) clones was analyzed with the use of a panel of syngeneic target cells infected with five heterologous influenza A strain viruses. Forty-five H-2 D-end-restricted CTL clones from B10.A(5R) responders (Dd,Ld) demonstrated 14 different patterns of recognition. Many of these clonotypes were a...

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In Vitro Expansion of Listeria Monocytogenes - Reactive T Lymphocyte Subsets in Humans - a Flow- Cytometry Approach

The incidence of human listeriosis is sporadic, but we previously demonstrated that T lymphocytes with reactivity to Listeria monocytogenes are with a high frequency in healthy individuals. As kinetics of specific expansion within CD4 and CD8 T cell compartments is particular to a certain pathogen, we attempted to describe the peculiarities which define the T cell reactivity to L. monocytogenes...

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Expression of specific cytolytic activity by H-2I region-restricted, influenza virus-specific T lymphocyte clones

Among murine class II major histocompatibility complex (MHC)-restricted cytotoxic T lymphocyte (CTL) clones specific for type A influenza virus, we have identified both noncytolytic clones and clones exhibiting H-2 I region-restricted cytolytic activity. After appropriate antigenic stimulation, both cytolytic and noncytolytic clones proliferated in the absence of exogenous interleukin 2. All of...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1986

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.164.1.363